2009～2010年华南地区副猪嗜血杆菌分离株ERIC-PCR指纹图谱的鉴定和分析

Identification and analysis of ERIC-PCR fingerprint of Haemophilus parasuis isolated from South China during 2009 and 2010

本试验采用肠杆菌基因间重复一致序列多态性聚合酶链式反应（ERIC-PCR）对2009~2010年分离自华南地区的41株副猪嗜血杆菌野生菌株及15株参考菌株进行了指纹图谱的鉴定。利用BioNumerics 5.1软件对所有菌株的ERIC-PCR指纹图谱进行分析,结果显示,15株具有不同血清型的参考菌株均具有不同的指纹图谱;41株华南地区副猪嗜血杆菌野生分离株则具有26个不同的指纹图谱,该方法对所有56株副猪嗜血杆菌的鉴别率为0.984。由此可见,ERIC-PCR分型方法具有较好种间的鉴别能力,可作为副猪嗜血杆菌病流行病学研究中的一种有效的辅助分子手段。

Enterobacterial repetitive intergenic consensus sequence based-PCR（ERIC-PCR） was used for characteration and genotyping of 56 Haemophilus parasuis strains including 15 reference strains and 41 clinical strains that were isolated from South China during 2009 and 2010.Analysis of the band patterns obtained by ERIC-PCR was performed with the BioNumerics 5.1 software package.The results displayed that Haemophilus parasuis reference strains of 15 different serovars have specific ERIC-PCR fingerprints respectively.Moreover,among the 41 clinical strains examined,a total of 26 different ERIC-PCR profiles were observed,and the discriminatory index of the ERIC-PCR is 0.984.The results showed that the ERIC-PCR assay is an efficient tool for molecular epidemiology research of Haemophilus parasuis.