α-双炔失碳酯对人卵巢癌细胞生长的影响及其与ER的关系

The effect of α-Anordrin on ovarian cancer cell lines and the relationship between the effect and ER

目的：探索α-双炔失碳酯对人卵巢上皮癌细胞体外生长的影响及其与ER的关系、方法：利用台盼蓝法、MTT法检测α-双炔失碳酯对两种ER含量不同的卵巢癌细胞株SKOV3、OVCAR-3的生长调节作用；光镜、电镜观察形态学变化、结果；（1）α-双炔失碳酯（6．25～100μmol／L）对ER含量低的OVCAR-3细胞的生长抑制效应强于ER含量高的SKOV3细胞，并呈浓度、时间依赖效应；（2）50μmol／Lα-双炔失碳酯作用72h后，两种细胞形态发生明显变化，细胞体积缩小呈梭形，核固缩。电镜下见胞浆内空泡结构增多，核糖体减少。结论：α-双炔失碳酯对卵巢癌细胞生长有一定抑制作用，该作用与ER的关系尚未完全肯定。

Objective To explore the effect of a-Anordrin on ovarian cancer cell lines and the relationship between the effect and ER. Methods: Two ovarian cancer cell lines SKOV3,OVCAR-3 which contain different amount of ER were chosen to asssy the inhibitory rate by typan blue dye exclusion and MTT assay. Morphic changes were demonstrated by microscopy and transmission electron microscopy. Results: (1)The proliferation of OVCAR3 cells which contain less ER was strongerly inhibited than SKOV3 cells by a-Anordrin,appearing a time and dosedepended way. (2)After a-Anordrin (50μmol/L)treatment for 72 hours,the morphism changed. Cells' volume reduced,swollon vesicles increased in cytoplasm and ribosomes decreased. Coclusions: a-Anordrin might have inhibitory effect on ovarian cancer cells growth,but the relationship between the effect and ER hadn't been confirmed.