白细胞介素10及受体在系统性红斑狼疮患者中表达的研究

Expressions of IL-10 and Its Receptor in Patients with Systemic Lupus Erythematosus

目的明确白细胞介素10(IL-10)及IL-10受体(IL-10R)在系统性红斑狼疮(SLE)发病过程中的作用。方法应用流式细胞微球捕获技术检测血浆IL-10表达水平,应用流式细胞方法检测外周血白细胞表面IL-10R表达水平。共检测40例SLE患者和20例正常对照者。以SLE疾病活动性指数(SLEDAI)判定SLE疾病活动性。结果狼疮肾炎(LN)患者血浆IL-10表达水平较正常对照者高。各细胞亚群表面的IL-10R表达水平在SLE和正常对照组中相似,但是,LN患者的CD4+和CD8+T细胞表面IL-10R表达降低。血浆IL-10水平与SLEDAI没有相关性,而CD4+T细胞和CD8+T细胞的IL-10R1表达水平与SLEDAI呈明显负相关。结论 IL-10及其受体在狼疮肾炎发病过程中可能具有重要的作用。

Objective Clarifying the role of IL-10 and its receptor IL-10R in the pathogenesis of human systemic lupus erythematosus（SLE）.Methods Flow Cytometric CBA method was used to detect plasma IL-10 levels.IL-10R1 expression levels on leukocytes were detected by Flow Cytometry.Expressions of IL-10 and IL-10R1 were detected in 40 SLE patients（20 with lupus nephritis and 20 without it） and twenty healthy controls.Results Plasma IL-10 levels were significant higher in SLE patients,especially in lupus nephritis（LN） patients.In addition,LN patients expressed significant lower IL-10R1 levels on CD4＋ and CD8＋ T cells,which were negatively correlated with SLE disease activity index（SLEDAI）.Conclusion IL-10 and its receptor may play an importan role in the pathogenesis of lupus nephritis.