胃癌中cyr-61、cox-2的表达及其临床病理学意义

Expression of cyr-61 and cox-2 in Gastric Carcinoma and Its Clinical Significance

目的检测胃癌组织及非瘤胃黏膜组织中富含半胱氨酸61蛋白(cyr-61)和环氧合酶2蛋白(cox-2)的表达水平,探讨二者表达的临床病理意义。方法应用免疫组织化学方法(S-P法)检测82例胃癌组织及43例非瘤胃黏膜组织中cyr-61、cox-2蛋白的表达。结果 82例胃癌中cyr-61蛋白高表达率为60.98%(50/82),非瘤胃黏膜组织中cyr-61蛋白的高表达率为34.88%(15/43),两者间差异有统计学意义(P<0.01);胃癌组织中cox-2的高表达率为62.20%(51/82),非瘤胃黏膜组织中cox-2高表达率为41.86%(18/43),差异亦有统计学意义(P<0.05)。其中胃癌淋巴结转移组cyr-61蛋白及cox-2蛋白高表达率均显著高于无淋巴结转移组(P<0.001);远距离转移组中cyr-61蛋白及cox-2蛋白高表达率均显著高于无远距离转移组(P<0.05);55例完整随访资料显示,cyr-61蛋白及cox-2蛋白高表达组5年生存率均显著低于低表达组5年生存率(P<0.001);cyr-61蛋白与cox-2蛋白在胃癌组织中的表达水平呈正相关关系(χ2=6.56,P<0.05)。结论 cyr-61、cox-2蛋白在胃癌组织中呈高表达,且表达水平与淋巴结转移、远距离转移、5年生存率密切相关,cyr-61、cox-2蛋白有可能成为胃癌预后的重要指标之一。

Objective To investigate the expression and the clinical significance of Cysteine-rich 61（Cyr-61/CCN1）and Cyclooxygenase-2（cox-2） in human gastric carcinoma.Methods The expression level of cyr-61 and cox-2 in 82 human gastric carcinoma and 43 non-tumor gastric mucosa tissues were detected by immunohistochemical staining.Results In the 82 gastric cancer,the incidence of high cyr-61 expression was 60.98%（50/82）,in contrast,the incidence in 43 non-tumor gastric mucosa tissues was 34.88%（15/43）,（P 〈 0.01）;The rate of high cox-2 expression was 62.20 %（51/82） in gastric cancer,in contrast,41.86%（18/43） in non-tumor gastric mucosa tissues.（P 〈 0.05）.In gastric cancer with lymph node metastasis,the incidence of high cyr-61 and cox-2 expression were both significantly higher than that of gastric cancer without lymph node metastasis（P 〈 0.001）.In gastric cancer with distance metastasis the incidence of high cyr-61 and cox-2 expression were both significantly higher than that of gastric cancer without distance metastasis,（P 〈 0.05）.55 complete data shown that in patients with high cyr-61,cox-2 expression,the 5-year overall survival rate were both significantly lower than that of low cyr-61,cox-2 expression patients,（P 〈 0.001）.The expression of cyr-61 was positive correlation with the expression of cox-2 in gastric cancer,（χ2 =6.56,P 〈 0.05）.Conclusion In gastric carcinoma,the expression of cyr-61 and cox-2 was high compare to non-tumor gastric mucosa tissues,and in patients with gastric cancer the expression level of cyr-61 and cox-2 was correlation with lymph node metastasis,distance metastasis,5-year overall survival rate.cyr-61 might be a prognosis marker for gastric carcinoma.