摘要
测定暴露于不同汞离子浓度下的罗非鱼(Tilapia niloticus)的肝脏碱性磷酸酶(alkaline phosphatase,AKP)活性,同时取其血液进行血细胞计数,制作血涂片、染色测定其微核细胞率和核异常率。结果表明:随着汞离子浓度的上升,罗非鱼的肝脏碱性磷酸酶活性下降;与对照相比,罗非鱼血细胞核异常率、微核率显著增加,白细胞数目增加,但红血细胞变化不大。表明罗非鱼外周血微核标记和肝脏碱性磷酸酶活性能够灵敏地指示水环境中的汞污染。
Effects of mercury on blood cells and alkaline phosphatase（AKP） activity in liver of were studied.Tilapia niloticus was randomly devided into 4 groups（three experimental groups and one control group,each group with 20 fish）.Experimental groups were exposed to different concentrations Hg2＋ concentrations（10,100,1 000 μg/L）.Then the blood cell and were counted and determined.The results showed that the AKP activity in liver decreased with the increment of Hg2＋ concentration,and were significant lower than that in control.Compared with the control,the nucleus abnormity rate and the micronucleus rate of Tilapia niloticus were increased significantly,the red blood cell no obvious change.The blood cells and AKP activity in liver can be used as indicators to evaluate Hg2＋ in water pollution examination.
出处
《广东农业科学》
CAS
CSCD
北大核心
2011年第13期114-117,F0003,共5页
Guangdong Agricultural Sciences
基金
广东省科技计划项目(2009B020308006)
广东省自然科学基金(9151064201000067)
广州大学华南生物多样性重点实验室项目(2010002)
关键词
汞
罗非鱼
碱性磷酸酶
血细胞
微核
mercury
tilapia niloticus
alkaline phosphatase（AKP）
bloods cells
micronucle
