摘要
随机选取同期孵化出雏的中国地方鸡种如皋鸡和引进隐性白羽鸡72只和55只,测定部分免疫性状。利用PCR-SSCP技术检测如皋鸡和隐性白羽鸡Nramp1基因第9外显子的多态性,并对不同基因型与免疫性状进行相关分析。结果表明:如皋鸡与隐性白羽鸡H/L、淋巴细胞转化率和IgM含量均差异显著(P<0.05)。如皋鸡AA型的H/L显著低于BB型和AB型(P<0.05),而AA型的淋巴细胞转化率和IgM含量显著高于BB型(P<0.05)。隐性白羽鸡各基因型对免疫指标的影响与如皋鸡的趋势相同。该研究结果初步显示,如皋鸡的综合免疫性能优于隐性白羽鸡,AA基因型的综合免疫性能优于AB型和BB型。
A total of 72 Rugao chicken（RG） and 55 Recessive White chicken（RW）,which belong to Chinese native chicken breeds and introduced breeds respectively,were selected randomly and incubated at the same stage.The value of immune traits was detected.PCR-SSCP technique was applied to analyze the correlation between the polymorphisms of Nramp1 gene and immune traits in RG and RW.The results showed thatthe heterophil/lymphocyte（H/L） value,lymphocyte transformation rate and the content of IgM of RG and RW were significantly different（P0.05）.H/L value of AA genotype was significantly lower than that of BB genotype and AB genotype,while the AA genotype＇s lymphocyte transformation rate and IgM were significantly higher than those of the BB genotype in both RG and RW.The results demonstrated that the general immune performance of RG was superior to that of RW.The general immune performance of AA genotype was superior to that of BB genotype and AB genotype.
出处
《中国畜牧杂志》
CAS
北大核心
2011年第15期5-8,共4页
Chinese Journal of Animal Science
基金
江苏省科技支撑计划(BE2010375)
江苏省科技支撑计划(BE2008359)
江苏省自然科学基金(BK2009190)
