RGS2基因C1114G多态性与阵发性房颤的相关性

Association between C1114G polymorphism of RGS2 gene and paroxysmal atrial fibrillation

目的分析G蛋白信号转导调节蛋白RGS2基因C1114G单核苷酸多态性与阵发性房颤(PAF)的相关性。方法选取PAF患者115例及健康对照者102例,应用创造酶切位点原理设计引物,采用PCR-RFLP技术检测RGS2基因C1114G等位基因型及等位基因频率分布。结果两组基因型频率均符合Hardy-Weinberg平衡(P>0.05)。两组间RGS2基因C1114G多态性的基因型频率及等位基因频率比较有统计学差异(P<0.01),其中GG基因型及1114G等位基因型频率在PAF患者中显著增高。结论 RGS2基因C1114G单核苷酸多态性与PAF相关,1114G等位基因可能是PAF的遗传易感基因。

Objecive To analyze the relation between C1114G polymorphism of RGS2 gene and paroxysmal atrial fibrillation. Methods A total of 115 subjects with paroxysmal atrial fibrillation were selected as case group and 102 healthy subjects as control group. PCR primers were designed by create restriction site（CRS）. The C1114G polymorphism of RGS2 was determined by polymerase chain reaction and restriction fragment length polymorphism（PCR-RFLP）. Results All observed genetype frequencies in both case group and control group were in Hardy-Weinberg equilibrium （ P 〉 0.05）. The differences of genetype frequencies and allele frequencies between two groups were significant （P 〈 0.01 ）, the GG genetype as well as the 1114G allele were significantly more common in the case group than control group. Conclusion The C1114G polymorphism of RGS2 may be associated with paroxysmal atrial fibrillation, 1114G allele is more susceptible to paroxysmal atrial fibrillation.