POD同工酶在酸枣、枣分类中的应用

A STUDY ON THE CLASSIFICATION OF JUJUBE AND WILD JUJUBE BY PEROXIDASE ISOZYME

运用聚丙烯酰胺凝胶电泳 ( PAGE)技术 ,对来自不同产地的 83个酸枣类型 (品种 ) ,及 36个枣品种的叶片过氧化物酶 ( POD)同工酶扫描数据 ,进行了主因子分析和聚类分析研究。结果表明 ,运用距离系统的类平均法 ( UPGMA)可将全部试材划分为六大类群和 3个特殊型 ,且聚类结果与地理位置直接相关。此外 ,依同工酶带的有无 ,可将全部供试枣品种叶片POD同工酶合并为 5种谱型 ,全部酸枣类型 (品种 )合并为 2 2种谱型。大马牙枣品种酶谱类型独特 ,初步推测其为供试酸枣和枣的中间型品种。

This test was carried out with polyacrylamide gel electrophoresis.Peroxidase isozyme data of eighty- three species of wild jujube and thirty- six varieties of jujube,which came from different places, were analysed with principal factor analysis and cluster analysis methods.The results showed that all the materials used in this testcould be classified into six clusters and three special types by unweighted pair- group mean average( UPGMA) method.And the classification results had direct relationship with geography.Meanwhile,five zymogram types of leaf peroxidase of cultivars were obtained according to the appearance of isozyme band,while all the wild forms had twenty- two.One of the jujube varieties named Da Maya had special zymogram type and was conjectured to be the transitional cultivar between cultivars and wild forms in this test.