蓝光对绿豆下胚轴愈伤组织形成和生长过程中蛋白质代谢的影响

Effects of Blue Light on Protein Metabolism During the Formation and Growth of Mung Bean Hypocotyl Calli

蓝光、白光和黑暗对绿豆下胚轴愈伤组织形成和生长过程中蛋白质代谢的影响不同。培养后３～１８ ｄ ，蓝光处理材料的可溶性蛋白质含量明显高于白光处理，更高于黑暗培养的材料。蓝光和白光明显促进３Ｈ亮氨酸掺入蛋白质，而蓝光和白光处理后游离氨基酸含量与黑暗对照相比，下降时间早，幅度大。在培养过程中，蛋白酶活性的变化与游离氨基酸相似。蛋白质合成抑制剂环己酰亚胺（ＣＨＭ） 抑制愈伤组织生长，其中以蓝光最大，白光次之，黑暗最小。在培养基中加入ＣＨＭ 愈早，抑制程度愈大。实验表明，ＣＨＭ 抑制愈伤组织蛋白质合成，也是以蓝光最甚。由此可见，蓝光促进绿豆下胚轴愈伤组织的形成、生长和蛋白质合成。

The effect of blue light on protein metabolism during the formation and growth of mung bean hypocotyl calli was investigated. The results obtained by measuring the protein contents, the incorporation of 3H leucine into protein and the proteinase activity demonstrated that 3 to 18 days after incubation, protein content in the explants grown under blue light was the highest (Fig.1). On the contrary, free amino acids in the tissue of blue and white light treatments declined faster and their contents were lower than those grown in the dark (Fig.3). The activity of proteinase was inhibited both by blue and white lights (Fig.4). Moreover, incorporation of 3H leucine into protein was strongly enhanced by blue and white light (Fig.2). The effectiveness of inhibiting callus growth by protein synthesis inhibitor CHM was in the order: blue light following by white light and darkness (Fig.5). The earlier the CHM added into medium, the greater the growth inhibition was observed (Fig.6). CHM inhibited the synthesis of protein in callus, particularly in that grown under blue light (Table 1). Hence, in compared with white light and darkness, blue light could promote the formation and growth of callus as well as protein synthesis.