摘要
建立了樱桃（PrunuspseudocerasusLindl．）矮化砧木茎尖高频再生系统，并利用根癌土壤杆菌（Agrobacteriumtumefaciens（SmithetTownsend）Conn）介导将gus及抗菌肽基因导入樱桃矮化砧木，获得19个转化株系。经Southernblot检测证明，抗菌肽基因已整合到樱桃的基因组。转基因植株接瘤实验、gus基因表达产物的颜色反应及叶片提取液对根癌土壤杆菌C58的抑菌实验表明，抗菌肽基因在转化植株中可能已得到有效表达，试管苗具有明显的抗根瘤病特性。还研究了茎尖分生组织的种质转化特点，结果表明它是一个较好的转化系统，可获得较高的转化频率。
A shoot regeneration system of high frequency from shoot of Chinese cherry (Prunus pseudocerasus Lindl. ) dwarfing rootstock was established and 19 transformed plants were obtained from the dwarfing rootstock by introducing gus gene and antibacterial polypeptide genes, using Agrobacterium tumefaciens (Smith et Townsend) Conn as mediator. The results of Southern blot analysis proved that antibacterial polypetide gene was integrated into the cheny genome. The results of tumor test, X-gluc reaction and bacterial-resistant test of leaf extract against Agrobacterium tumefaciens C58 indicated that antibacterial polypeptide gene may be expressed efficiently in the transformed plant- The tube shoot was chararteristic in its resistance to root nodule disease. This study on the charateristics of germ line transformation of shoot meristem suarested that it was a highly efficient transformaion system.
基金
辽宁省国家地方攻关资助!97-21-04
