花生四烯酸5-脂氧合酶激活蛋白基因多态性与急性冠状动脉综合征的易感性

Arachidonate 5-lipoxygenase activating protein gene SG13S114T/A polymorphism and the susceptibility to acute coronary syndrome

目的探讨花生四烯酸5脂氧合酶激活蛋白(ALOX5AP)基因SG1 3S114T/A多态性与急性冠状动脉综合征(ACS)的易感性。方法选择住院的胸痛患者714例,将确诊为ACS的患者377例作为ACS组,非ACS患者337例作为对照组,采用聚合酶链反应限制性片段长度多态性方法检测ALOX5AP基因SG13S114T/A多态性,并进行logistic回归分析。结果 ACS组患者AA、AT和TT基因型频率分别为1 3.79%、50.93%和35.28%,对照组患者分别为12.76%、38.58%和48.66%,2组AT和TT基因型频率差异有统计学意义(P=0.041,0.020);ACS组男性AT基因型频率高于对照组(P=0.040),女性TT基因型频率低于对照组(P=0.013)。SG13S114T/A位点AT和TT基因型以及T等位基因是所有ACS(P=0.004、0.001和0.013)和男性ACS(P=0.014、0.005和0.020)发病的危险因素。结论 ALOX5AP基因SG1 3S114T/A多态性AT和TT基因型以及T等位基因可能与老年人,特别是老年男性ACS的易感性相关。

Objective To investigate the possible association of arachidonate 5-lipoxygenase activating protein（ALOX5AP）gene SG13S114T/A polymorphism with the susceptibility to acute coro nary syndrome（ACS）. Methods 714 in patients with chest pain were chosen and were divided into ACS group （377 cases with ACS） and control group （337 subjects without ACS）. The ALOXSAP gene SG13S114T/A polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphism analysis, and multiple logistic regression analysis was performed. Results Genotype frequencies of ALOXSAP gene SG13S114T/A AA, AT and TT were 13.79%,50. 93% and 35.28% respectively in patients of ACS group and 12.76%,38.58% and 48.66% respectively in control subjects. Compared with control group, there was statistical difference in frequencies of AT and TT genotypes in ACS group （P = 0. 041,0. 020）. The AT genotype frequency in male ACS group was obeviously higher（40.7% vs 52.5%, P = 0. 040） and the TT genotype frequency in female ACS group was significantly lower（61.8% vs 37.0%, P = 0. 013） than control group. The frequencies of AT,TT genotypes and the T allele were related with attack of ACS （P = 0. 004,0. 001 and 0. 013,respectively） and male ACS （P = 0. 014,0. 005 and 0. 020,respectively）. Conclusion The AT and TT genotypes and the T allele of ALOXSAP gene SG13S114T/A polymorphism may be associated with the susceptibility to ACS in the elderly,especially in the aged males.