摘要
目的:建立测定蝙蝠葛碱的高效液相色谱(HPLC)法,并研究蝙蝠葛碱与大鼠和人血浆蛋白的结合率。方法:采用平衡透析法,HPLC法测定蝙蝠葛碱的浓度,计算蝙蝠葛碱大鼠及人血浆蛋白结合率。结果:透析外液中的蝙蝠葛碱检测浓度在0.24~15.10μg.mL-1范围内与其峰面积积分值呈良好线性关系;大鼠血浆中的蝙蝠葛碱检测浓度在0.24~15.10μg.mL-1范围内与其峰面积积分值呈良好线性关系;人血浆中的蝙蝠葛碱检测浓度在0.24~15.10μg.mL-1范围内与其峰面积积分值呈良好线性关系。蝙蝠葛碱在高、中、低浓度下,其大鼠血浆蛋白结合率分别为(69.63±1.30)%、(68.64±1.10)%、(72.54±0.50)%,人血浆蛋白结合率分别为(82.1±1.00)%、(84.96±0.70)%、(78.79±0.60)%。结论:HPLC法用于蝙蝠葛碱生物样品测定具有简便、灵敏与选择性高的特点;蝙蝠葛碱具有较强的血浆蛋白结合率,且大鼠和人血浆蛋白结合率具有种属差异性,人血浆蛋白结合率高于大鼠血浆蛋白结合率(P<0.05)。
OBJECTIVE:To establish the HPLC method for the determination of dauricine,and to study the plasma protein binding rate of it.METHODS:The equilibrium dialysis and HPLC were used to determine the concentration of dauricine,and rat and human plasma protein binding rate of dauricine was calculated.RESULTS:The linear range of dauricine in extracellular fluid microdialysis was 0.24~15.10 μg·mL-1;in rat plasma was 0.24~15.10 μg·mL-1;in human plasma was 0.24~15.10 μg·mL-1;At high,middle and low concentrations,the rat plasma protein binding rates of dauricine were(69.63±1.30)%,(68.64±1.10)%,(72.54±0.50)%,respectively.The human plasma protein binding rates of dauricine were(82.1±1.00)%,(84.96±0.70)%,(78.79±0.60)%,respectively.CONCLUSIONS:HPLC method is simple,sensitive and selective for the determination of biological sample of dauricine.The plasma protein binding rate of dauricine is of a little high,and the human protein binding rate is higher than that of rat,there is difference among species(P0.05).
出处
《中国药房》
CAS
CSCD
北大核心
2011年第31期2894-2897,共4页
China Pharmacy