不同产地金银花与山银花主要成分的含量比较

Content Comparison of Main Compounds in Lonicera japonica and Flos Lonicerae from Different Producing Areas

目的:比较不同产地金银花与山银花中主要成分含量的差异。方法:采用高效液相色谱(HPLC)-紫外扫描(UV)法测定不同产地金银花和山银花中绿原酸和木犀草苷的含量;采用HPLC-蒸发光散射检测器(ELSD)法测定2种药材中灰毡毛忍冬皂苷乙和川续断皂苷乙的含量。色谱柱为Agilent ZORBAX SB-C18(250mm×4.6mm,5μm),测定木犀草苷的流动相为乙腈-0.2%磷酸(梯度洗脱),流速为1.0mL·min-1,检测波长为350nm;测定绿原酸、灰毡毛忍冬皂苷乙和川续断皂苷乙的流动相为0.4%冰醋酸-乙腈(梯度洗脱),流速为1.0mL·min-1,绿原酸检测波长为324nm,其余两成分采用ELSD。结果:不同产地金银花中绿原酸、木犀草苷含量分别为2.227%～2.931%、0.0389%～0.0739%,不含灰毡毛忍冬皂苷乙和川续断皂苷乙;不同产地山银花中绿原酸含量为3.039%～5.657%,灰毡毛忍冬皂苷乙和川续断皂苷乙的总含量为5.59%～9.29%,不含木犀草苷,均符合现行版药典规定。结论:本试验结果可为金银花人工培育和GAP基地建设提供依据,同时为有效鉴别制剂中的金银花和山银花提供依据。

OBJECTIVE：To compare the difference of main compounds in Lonicera japonica and Flos Lonicerae from producing areas.METHODS：The contents of chlorogenic acid and galuteolin in L.japonica and Flos Lonicerae from producing areas were determined by HPLC-UV.The contents of macranthoidin B and dipsacoside B were determined by HPLC-ELSD.The Agilent ZORBAX SB-C18（250 mm×4.6 mm,5 μm） was adopted.The mobile phase consisted of acetonitrile-0.2% phosphoric acid（gradient elution） for galuteolin at flow rate of 1.0 mL·min-1 with detection wavelength of 350 nm.The mobile phase consisted of 0.4% glacial acetic acid-acetonitrile（gradient elution） for chlorogenic acid,macranthoidin B and dipsacoside B at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 324 nm for chlorogenic acid,and the other was determined by ELSD.RESULTS：The contents of chlorogenic acid and galuteolin in L.japonica were 2.227%～2.931% and 0.038 9%～0.073 9%,excluding macranthoidin B and dipsacoside B;The total contents of macranthoidin B and dipsacoside B in Flos Lonicerae were 5.59%～9.29%.Galuteolin was not found.They were all in line with the standards stated in current pharmacopeia.The contents of chlorogenic acid were 3.039%～5.657%.CONCLUSION：The study provides references for artificial cultivation of L.japonica and GAP base construction,and it also provides basis for the identification of L.japonica and Flos Lonicerae.