HPLC法测定复方银连颗粒中绿原酸的含量

Content Determination of Chlorogenic Acid in Compound Yinlian Granules by HPLC

目的:建立测定复方银连颗粒中绿原酸含量的方法。方法:采用高效液相色谱法。色谱柱为Welchrom C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸(10∶90),检测波长为327nm;流速为1.0mL·min-1,柱温为30℃。结果:绿原酸进样量在0.3035～1.5175μg范围内与峰面积积分值呈良好线性关系(r=0.9996);平均回收率为101.6%,RSD=1.84%(n=6)。结论:本方法操作简便、可靠,可用于复方银连颗粒的质量控制。

OBJECTIVE：To establish the method for the content determine of chlorogenic acid in Compound yinlian granules.METHODS：HPLC method was adopted.The Welchrom C18 column（250 mm×4.6 mm,5 μm）was used with mobile phase consisted of acetonitrile-0.1% phosphoric acid（10：90）.The detection wavelength was set at 327 nm and flow rate was 1.0 mL·min-1.The column tempareture was 30 ℃.RESULTS：The calibration curve of baicalin was linear within the range of 0.303 5～1.517 5 μg（r=0.999 6）.The average recovery was 101.6%（RSD=1.84%,n=6）.CONCLUSION：The method is simple,convenient and reliable.It is appropriate for the quality control of Compound yinlian granules.