丹皮酚对心肌细胞自律性和延迟后除极的影响

EFFECTS OF PAEONAL ON AUTOMATICITY AND AFTERDEPOLARIZATION OF GUINEA PIG PAPILLARY MUSCLES

目的与方法：采用常规玻璃微电极技术研究丹皮酚对离体心肌细胞自律性（ＡＭ）、延迟后除极（ＤＡＤ） 及触发活动（ＴＡ）的影响。结果：１．８×１０－４ｍｏｌ／Ｌ丹皮酚灌流组，肾上腺素（Ａｄｒ）的阈浓度空白对照组为（１．２８±０．５７）μｍｏｌ／Ｌ，药后为（１．５６±０．５３）μｍｏｌ／Ｌ（ｎ＝９，Ｐ＞０．０５）；用（１．８×１０－ ３） ｍｏｌ／Ｌ丹皮酚（Ｐａｅ）灌流组，Ａｄｒ 浓度由空白对照组的（１．２２ ±０．６２）μｍｏｌ／Ｌ升高到（６．２２±２．１１）μｍｏｌ／Ｌ（ｎ＝９，Ｐ＜０．０１）。１．８×１０－３ｍｏｌ／Ｌ的Ｐａｅ 能明显抑制哇巴因（Ｏｕａ）诱发的ＤＡＤ的幅值，当基本刺激周长为５００，４００，３００ 和２００ ｍｓ 时，其ＤＡＤ幅值从（５．５±２．０）ｍＶ，（７．３±２．１）ｍＶ，（８．０ ±２．４）ｍＶ和（９．２±１．９）ｍＶ减小到（３．０±１．１）ｍＶ、（３．６±１．７）ｍＶ，（４．３±２．０） ｍＶ和（５．９ ±１．６） ｍＶ，Ｐ＜０．０１。当基本刺激周长为２００ ｍｓ时，ＴＡ 数目由５．５±１．０ 降至０．７±０．３（Ｐ＜０．０１）。结论：丹皮酚能抑制心肌细胞ＡＭ、ＤＡＤ及ＴＡ。

Aim and Methods: Standard microelectrode technique was used to study the effects of paeonal on the automaticity induced by epinephrine, ouabain induced delayed afterdepolarization (DAD) and triggered activity (TA) of isolated guinea pig right ventricular papillary muscle. Results: Paeonal 1.8×10 -3 mol/L could inhibit the automaticity, concentration of epinephrine from (1.22± 0.62 ) μmol/L to (6.22±2.11)μmol/L ( n =9, P <0.01), but paeonal 1.8×10 -4 mol/L exected no influence on the automaticity, concentration of epinephrine from 1.28±0.57 μmol/L to 1.56±0.53μmol/L ( n =9, P >0.05). In additional presence of paeonal 1.8×10 -3 mol/L, the DAD amplitude at 500 ms, 400 ms, 300 ms and 200 ms were decreased from 5.5±2.0, 7.3±2.1, 8.0± 2.4 and 9.2±1.9 to 3.0±1.1, 3.6±1.7,4.3±2.0 and 5.9±1.6(mV) respectively, all P <0.01. The number of TA induced at BCL 200 ms was decreased from 5.5±1.0 to 0.7±0.3( P <0.01). Conclution:Paeonal could inhibit automaticity, DAD and TA, which contributes to its anti arrhythmic effect.