福尔马林引起的急性内脏炎症痛模型

A MODEL OF FORMALIN INDUCED ACUTE VISCERAL INFLAMMATARY PAIN

目的：为深入开展内脏痛的研究，建立急性内脏炎症痛模型。方法：实验选用Ｗｉｓｔａｒ 大鼠，雌雄不拘，体重２４０～２７０ ｇ。本实验分两个部分：①大鼠乙状结肠壁粘膜下注射福尔马林（０ ．１ｍｌ，５ ％） 复制急性内脏炎症痛模型；②检测吗啡（１ ｍｇ／ｋｇ，２ ｍｇ／ｋｇ，４ ｍｇ／ｋｇ）对模型疼痛分数的影响。结果：①福尔马林具有显著的致痛效应，实验组的疼痛分数显著高于对照组（ Ｐ＜ ０．０１） ；②疼痛分数能够反映内脏疼痛程度；③吗啡对模型具有显著、剂量依赖的止痛效应（Ｐ＜ ０．０１） 。结论：①大鼠乙状结肠壁粘膜下注射福尔马林可作为急性内脏炎症痛模型；②疼痛分数能客观反映了内脏疼痛程度。

Aim:Making a model of visceral pain is an indispensable step to further the research about visceral pain.Methods:The work included two parts:①An acute local visceral pain model was produced by injection of formalin submucosally (0.1 ml, 5%) and the degree of pain was evaluated and expressed as pain scores (S).②Morphine (1 mg/kg, 2 mg/kg, 4 mg/kg) was injected intraperitoneally and the effects of morphine on the pain scores were measured.Results:①Formalin had a significant algesic effect and the pain scores of experimental group were significantly higher than those of the control groups ( P <0.01).②Pain scores can reflected the degree of visceral pain.③Morphine had a significant and dose dependant analgesic effect in the model( P <0.01).Conclusions:①A model of visceral inflammation pain can be produced by sigmoid submucosal injection of formalin.② Pain scores can objectively evaluate the degrees of visceral pain as a quantitating index.