电化学极化玻碳电极构建的葡萄糖生物传感器

Biosensor for glucose based on electrochemical polarization glassy carbon electrode

在硝酸溶液中电化学极化处理玻碳电极（GCE）,以硫堇（TH）为电子介体,通过金—硫、金—氮共价键作用和静电吸附作用将纳米金（GNPs）、葡萄糖氧化酶（GOD）和辣根过氧化物酶（HRP）修饰于电极上,最后在电极上滴涂Nafion水溶液制备抗干扰膜并固定酶电极,构建了一种新型双酶葡萄糖生物传感器。实验结果表明：电化学极化处理电极提高了生物传感器的性能,传感器响应电流与葡萄糖浓度在1×10-3~18×10-3mol/L范围内有良好的线性关系,R=0.991 7,检出限为5×10-5mol/L。该生物传感器具有良好的灵敏度、抗干扰性和稳定性,可以重复使用。

A novel amperometric bienzymatic biosensor for determination of glucose is developed by modifying thionine（TH）film on the electrochemical pretreated glassy carbon electrode（GCE）surface.Gold nanoparticles（GNPs）,glucose oxidase（GOD）and horseridesh peroxidase（HRP）are immolilized by electrostatic adsorption and Au-S,Au-N covalent bond self-assembly.Nafion solution is dropped on the modified electrode to prepare anti-interferential and fixed membrane.The experimental results show that the performance of the biosensor can be improved by the electrochemical polarization glassy carbon electrode in HNO3 solution.A good linear relationship between response current and glucose concentration in the range of 1×10-3~18×10-3mol/L,R=0.991 7,the detection limit is 5×10-5mol/L.The biosensor has high sensitivity,long-term stability,excellent interference immunity and reusability.