人参总皂苷对脑外伤大鼠脑组织氧化应激指标的影响

Effect of ginseng total saponins on oxidative stress variables in the brain tissue after traumatic brain injury in rats

目的观察人参总皂苷对脑外伤大鼠损伤灶周围脑组织中一氧化氮(NO)和丙二醛(MDA)含量及一氧化氮合酶(NOS)和超氧化物歧化酶(SOD)活性的影响,探讨其抑制创伤性脑损伤(traumatic brain injury,TBI)后继发性损伤的作用机制。方法运用改良Feeney法建立大鼠脑外伤模型,将动物随机分为假手术组、脑外伤组、人参总皂苷治疗组。24 h后处死,采用干湿重法测量脑水含量,尼氏染色光镜下观察海马细胞形态,测量脑组织中NO、MDA的含量和NOS、SOD的活性。结果与脑外伤组相比,人参总皂苷治疗后脑水含量明显减低,损伤侧海马病理学改变明显减轻,脑组织中SOD活性明显上升,NO、MDA的含量和NOS的活性明显下降(P<0.01)。结论人参总皂苷可以减轻脑外伤后继发性损伤,其机制可能与提高脑组织中SOD活性,降低NOS活性,减少NO、MDA的生成,抑制氧化应激反应有关。

【Objective】 To observe the effect of ginseng total saponins（GTS） on NO,NOS,SOD,and MDA in the brain tissue after traumatic brain injury（TBI） in rats,and to explore the underlying mechanisms of GTS treatment on the secondary brain injury.【Methods】 Rats were randomly divided into 3 groups： sham-operated group,TBI group and GTS-treated group.TBI model was established with the modified Feeney＇s method.24h after TBI,the rats were sacrificed to measure the brain water content with wet-dry weight method.The morphological change of neuron in hippocampal area was observed under optical microscope through Nissl staining.The contents of NO and SOD,and the activity of NOS and SOD in the brain tissue were detected with biochemistry methods.【Results】 Compared with TBI group,the water content of brain was decreased,the damage of hippocampal area was alleviated,the activity of SOD was elevated,and the content of NO and MDA and the activity of NOS were reduced significantly（P 0.01） in GTS-treated group.【Conclusion】 Ginseng total saponins could exert a neuroprotective effect in rats after traumatic brain injury.The underlying mechanism of this effect is potentially related to elevating the activity of SOD and reducing the activity of NOS and the content of NO and MDA in the brain tissue.