荧光光谱法研究6-巯基嘌呤的氧化反应及其分析应用

A Study on the Redox Reaction of 6-Mercaptopurine by Spectrofluorimetry and Its Analytical Application

系统地研究了高锰酸钾对 6 巯基嘌呤的氧化作用 ,提出了高灵敏测定 6 巯基嘌呤的荧光光度分析新方法。在碱性介质中 ,氧化产物的荧光强度较 6 巯基嘌呤自身的荧光强度提高了 1 0倍 ,且稳定性好。本体系的激发波长为 2 86nm,发射波长为 397nm,荧光强度与 6 巯基嘌呤的浓度在 0 .0 0 64～ 3.0μg m L范围内呈线性关系 ,检测限为 0 .0 0 32μg m L。应用该法于片剂中 6 巯基嘌呤含量的测定 ,与标准方法对照 ,结果令人满意。

A new redox reaction spectrofluorimetry for the determination of 6 mercaptopurine (6 MP) in tablets was developed. The reaction proceeded completely in the presence of 0.16 mol·L -1 sodium hydroxide and 1.2×10 -4 mol·L -1 potassium permanganate. The fluorescence was measured at 397 nm with excitation at 286 nm. The calibration curve was linear over the concentration range 0.0064～3 μg/mL for 6 MP. The method has been applied to the determination of the compounds either in pure form or in tablets. The results obtained were in agreement with those given by an official method.