N-羟基琥珀酰亚胺-间二甲氨基苯甲酸酯柱前衍生HPLC分离荧光检测胺的研究

Separation and Fluorescence Determination of Amines as Their N-Succinimidyl-m-Dimethylphenylformate Precolumn Derivatives by HPLC

用 N 羟基琥珀酰亚胺 间二甲氨基苯甲酸酯为柱前衍生试剂 ,C18柱 ,含 p H为4 .0的 1 0 mmol L柠檬酸 磷酸氢二钠缓冲溶液的 4 0 %的甲醇 水 ( V V)溶液为流动相 ,反相高效液相色谱 ( RP HPLC)分离荧光检测了氨、甲胺和乙胺 ,检出限分别为 5 .0、0 .5和1 .0 pmol,方法简便、快速。

This paper reports the separation and fluorescence determination of ammonia, methylamine and ethylamine by reversed phase HPLC using N succinimidyl m dimethylphenylformate as a precolumn derivatization reagent. On a C 18 column, the derivatives of three compounds are separated on a baseline with the mobile phase of methanol water (40∶60, V/V ) containing citric acid Na 2HPO 4 (pH4.0,10 mmol/L). The detection limits of NH 3, CH 3NH 2 and CH 3CH 2NH 2 are 5.0, 0.5 and 1.0 pmol, respectively.