大口径毛细管气相色谱法测定罂粟壳中的生物碱

Determination of Alkaloids in Pericarpium Papaveris by Gas Chromatography with Wide Bore Capillary Column

采用超声提取大口径毛细管气相色谱法同时测定罂粟壳中可待因、吗啡、蒂巴因、罂粟碱和那可汀的质量比，用ＨＰ－１（５ ｍ ×０．５３ｍ ｍ ×２．６５ μｍ ）毛细管柱，ＧＣ－ＦＩＤ测定，结果表明，可待因、吗啡、蒂巴因、罂粟碱和那可汀的平均回收率分别为９４．０ ％ ，９６．５％ ，９３．８％ ，９１．０％ ，９１．４％ ， ＲＳＤ＝ ０．９２％ ～２．７５％ 。

A method for the simultaneous determination of codeine, morphine, thebaine, papaverine and narcotine in pericarpium papaveris by gas chromatography with wide bore capillary column is described. The major components in pericarpium papaveris were extracted with methanol and chloroform by supersonic extraction and evaporation. The residue was dissolved in methanol. The resulting solution was used for analysis. The conditions for determination were:FID detector, HP 1 capillary column, 5 m×0.53 mm×2.65 μm film , column temperature 260 ℃,The method requires10minutes for the whole analysis. The average recoveries of codeine, morphine, thebaine, papaverine and narcotine in the sample were 94.0%,96.5%, 93.8% ,91.0% and 91.4% respectively. The relative standard deviations were from 0.92% to 2.75%. The advantages of this method are simple, rapid, accurate and sensitive.