用HPLC法同时测定混合乳油“草敌”中的灭草松和2,4-滴丁酯

Determination of Bentazon and 2,4-D Butyl in Mixture Formulation by HPLC

用反相高效液相色谱法同时测定了新型复配除草剂草敌的有效成分——灭草松和２，４－滴丁酯。选择甲醇／乙腈／柠檬酸缓冲溶液为流动相，测得灭草松的线性相关系数为１．０００，回收率为（９８．６±０．９）％ ，２，４－滴丁酯的线性相关系数为１．０００，回收率为（１００．２±０．８）％ 。

Bentazon and 2,4\|D butyl(the active ingredients of Cao Di) were determined by reversed phase HPLC aimultancously using methanol/acetonitrile/citrate buffer(pH 3.1) as a mobile phase on Shim\|pack clc\|CN column. Results showed that the linear correlation and recovery of 2,4\|D butyl were 1.000 and ( 100.2 ±0.8)% respectively, and those of bentazon were 1.000 and (98.6±0.9)% respectively. The coefficients of variation for bentazon and 2,4\|D butyl were all was 0.37%.