摘要
建立了测定烟草中β－胡萝卜素的高效薄层色谱扫描方法，以Ｖ（苯）ｖＶ（丙酮）＝ ４ｖ１为展开剂，检测波长４５０ ｎｍ ，线性范围５０ ｎｇ～０．５ μｇ，最低检测限２０ ｎｇ。方法简便、灵敏、快速、重现性好。
This paper reports the high performance thin\|layer chromatography (HPTLC) determination of β \|carotene in tobacco. The developer used was V (benzene)∶ V (acetone)=4∶1.The detection wavelength was 450 nm. The linear range and the detectable limit of the method were 50 ng\|0.5 μg and 20 ng per spot of β \|carotene respectively. The method has been proved to be simple, sensitive,accurate and reproducible.
出处
《色谱》
CAS
CSCD
北大核心
1999年第6期606-607,共2页
Chinese Journal of Chromatography
关键词
高效薄层色谱
胡萝卜
烟草
测定
high performance liquid chromatography
β \|carotene
tobacco
