牛种布鲁杆菌PCR检测方法的研究

PCR method for detection of Brucella

为了弥补血清学和微生物学方法检测和诊断布鲁菌病的种种不足,建立用布鲁菌BCSP31聚合酶链反应(PCR)快速检测布鲁菌病原的方法。根据编码牛种布鲁菌31 000外膜蛋白基因序列设计的一对特异性引物,扩增出预期350bp的基因片段。结果,此方法具有较高的特异性和敏感性,DNA最低检测量为0.42pg。结果表明,布鲁菌病原PCR快速检测方法的建立,对布鲁菌病的检疫及快速诊断具有十分重要的意义。

To make up for various deficiencies of serology and microbiology method for detection and diagnosis of Brucellosis,the study established polymerase chain reaction（PCR） for rapid detection of Brucellosis.According to encoding bovine species Brucella 31 000 outer membrane protein gene sequence,designed a specific primer,amplified expected 350 bp fragment of the gene.A high specificity and sensitivity of PCR method was made up,the minimum detection limit for DNA 0.42 pg.The PCR method could be used for Brucellosis quarantine and diagnosis.