异噻唑啉酮类化合物对三角褐指藻的抑制效应

The inhibitory effect of isothiazolones on Phaeodactylum tricornutum

研究了不同浓度异噻唑啉酮类化合物BIT（1,2-苯并异噻唑啉-3-酮）及其类似物X（N-丙酰基-1,2-苯并异噻唑啉酮-3-酮）对三角褐指藻生长的抑制效应.根据藻细胞生长、比生长速率、藻细胞密度比、藻细胞内色素含量变化结果表明,在实验所设定的浓度（0-3mg/L）范围内,BIT和X在高浓度下对三角褐指藻生长均具有一定的抑制效应,增加细胞生长的延滞期,但在低浓度下不明显,BIT的抑制效应优于X,但BIT和X的这种抑制作用均会随着处理时间的延长而减弱,藻细胞逐渐恢复快速增殖.根据Logistic曲线方程拟合获得的BIT和X对藻的96h半效应质量浓度（EC50）分别为1.95mg/L（R^2=0.988,P=0.0013）和3.26mg/L（R^2=0.908,P=0.0279）,EC50值的大小进一步说明BIT比X对三角褐指藻的抑制作用强.

The growth inhibition effect of isothiazolone BIT（1,2-benzo-isothiazolin-3-ketone） and its analogue X（N-propionyl-1,2-benzo-isothiazolone ketone-3-ketone） with different concentrations on Phaeodactylum tricornutum were studied.The results of algal cells growth,specific growth rate,algal cells density ratio indicated that the growth of Phaeodactylum tricornutum could be inhibited at the initial growth phase after the algal cells were treated with high concentrations BIT and X.However the treatment groups with low concentrations BIT and X were not obvious compared with the control.The inhibitory effect of BIT was stronger than X,but the effect could decrease with the treatment time prolonged,the algal cells will proliferate rapidly.The EC50values of BIT and X were 1.95mg/L（R^2 = 0.988,P = 0.0013） and 3.26mg/L（R^2 = 0.908,P= 0.0279） respectively according to the Logistic curve fitting equation.It further showed that BIT had the better inhibitory effect than its analogue X.