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HPLC分离黄曲霉毒素生防微生物的拮抗物及拮抗效果检测 被引量：1

Purify the antagonists of biological-control agent against aflatoxin by HPLC and detect their inhibitive effect
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摘要 黄曲霉毒素在世界范围内不仅带来巨大的经济损失,而且严重威胁人们的身体健康。生物防治技术在黄曲霉毒素防治方面具有一定优势。利用课题组从土壤中筛选出的一株黄曲霉毒素生防细菌B05,使用HPLC对其代谢产物进行初步分离,确定了分离条件。将分离物收集后通过微孔板培养,确定了各分离组分对黄曲霉毒素的拮抗效果。 In the world aflatoxins lead to great economy loss and seriously endanger human health.The biological-control method has advantage on inhibiting aflatoxins.B05 isolated from soil was used as biological-control agent against aflatoxins.The antagonists of biological-control agent was purified by using HPLC and the purification condition was confirmed.The isolates were collected and their inhibitive effect was detected by microtiter agar plate assay.
作者 王凯 闫培生 王琢 任秀莲 郇征宇
机构地区 哈尔滨工业大学(威海)海洋学院 哈尔滨工业大学市政环境工程学院
出处 《食品科技》 CAS 北大核心 2011年第8期32-34,共3页 Food Science and Technology
基金 国家自然科学基金项目(30571244 30870003) 哈尔滨工业大学科研创新基金项目(IMXK57080022)
关键词 黄曲霉毒素 生物防治 拮抗物 HPLC 线性梯度洗脱 aflatoxin biological-control antagonist HPLC gradient elution
分类号 TS201.3 [轻工技术与工程—食品科学]
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参考文献5

  • 1Dorner J W, Cole R J, Blankenship P D. Use of a biocompetitive agent to control prehavest aflatoxin in drought stressed peanuts[J]. Food Prot,1992,(55):888-892.
  • 2Pitt J I, Hocking A D. Mycotoxins in Australia: biocontrol of aflatoxin in peanuts[J]. Mycopathologia,2006,162(3):233- 243.
  • 3N Boyraz, M Ozcan. Antifungal effect of some spice hydrosols[J]. Fitoterapia,2005,(76):661-665.
  • 4VA Nesci, RV Bluma, MG Etcheverry. In vitro selection of maize rhizobacteria to study potential biological control of Aspergillus section Favi and aflatoxin production [J]. Plant Patholo,2005,113 (2): 159-171.
  • 5闫培生,曹立新,王凯,王琢.真菌毒素生物防治研究进展[J].中国农业科技导报,2008,10(6):89-94. 被引量：15

二级参考文献30

  • 1Egnond H P, Jonker M A. Current situation on regulations for mycotoxins [ A ]. In : Yoshizawa T. ( ed. ). Proceedings of the International Symposium of Mycotoxicology [ C ]. Japan : Bikohsha, 2004,1 - 16.
  • 2Gareis M. Ochratoxin A-sources of exposure and risk assessment [ A ]. In: Yoshizawa T. ( ed. ). Proceedings of the International Symposium of Mycotoxicology [ C ]. Japan : Bikohsha, 2004, 49 - 58.
  • 3Simpson D R, Weston G E, Turner J A, et al.. Differential control of head blight pathogens of wheat by fungicides and consequences for mycotoxin contamination of grain [ J ]. Euro. J. Plant Pathogens, 2001,107:421 - 431.
  • 4Brown R L, Cotty P J, Cleveland T E. Reduction in aflatoxin content of maize by atoxigenic strains of Aspergillus flavus [ J ]. J. Food Prot. , 1991,54:623 -626.
  • 5Cotty P J. Effect of atoxigenic strains of Aspergillus flavus on aflatoxin contamination of developing cottonseed [ J ]. Plant Dis. , 1990, 74:233 -235.
  • 6Dorner J W. Biological control of aflatoxin crop contamination [A]. In: Abbas H K. (ed.). Aflatoxin and Food Safety [C]. Boca Raton, FL: CRC Press, 2005, 333 -352.
  • 7Pitt J I, Hocking A D. Mycotoxins in Australia: biocontrol of aflatoxin in peanuts [ J ]. Mycopathologia, 2006, 162: 233 - 243.
  • 8Dorner J W , Cole R J, Blankenship P D. Use of a biocompetitive agent to control prehavest aflatoxin in drought stressed peanuts [J]. J. Food Prot. , 1992, 55:888-892.
  • 9Dorner J W, Cole R J, Wicklow D T. Aflatoxin reduction in corn through field application of competitive fungi [ J ]. J. Food Prot. , 1999, 62:650 - 656.
  • 10Dorner J W, Cole R J. Effect of application of nontoxigenic strains of Aspergillus flavus and A. parasiticus on subsequent aflatoxin contamination of peanuts in storage [ J ]. J. Stored Products Res. , 2002, 38:329 - 339.

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食品科技
2011年 第8期

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