发芽糙米中γ-氨基丁酸的HPLC分析方法

High-performance liquid chromatography for determination of γ-aminobutyric in sprout brown rice

以2,4-二硝基氟苯为衍生试剂,以0.05mol/L乙酸钠缓冲液(pH6.5,含10mLN,N-二甲基甲酰胺)和50%乙腈为流动相,建立了发芽糙米中γ-氨基丁酸含量的高效液相色谱分析方法,色谱柱为SB-C18,梯度洗脱,紫外检测波长为360nm。γ-氨基丁酸的线性范围在10～250mg/L,峰面积与浓度之间线性关系良好,线性方程为Y=225.53095X-0.660345,R2=0.99956,RSD为0.28%,加标回收率为94.4%～98.2%。该方法操作步骤简单,所用试剂价格低,稳定、准确。

To establish a HPLC method for determining the contents of γ-aminobutyric acid in sprout brown rice,a SB-C18 column was used with the mobile phase of 50% methyl cyanide and 0.05 mol/L sodium acetate buffer solution which pH was 6.5 and contained 10 mL 2,4-dinitro-1-fluorobenzene,the detection wavelength of 360nm and gradient elution.The assay was linear from 10 to 250 mg/L.The linear equation between peak area and concentration was Y=225.53095X-0.660345 which R2=0.99956 and RSD was 0.28%.The recovery rate in standard addition calibration technique was from94.4% to 98.2%.This HPLC method is simple,economic,stable and accurate with good reproducibility for the contents of γ-aminobutyric acid.