丛生盔形珊瑚共附生可培养真菌多样性分析

Phylogenetic diversity analysis of cultured symbiotic fungi of Galaxea fascicularis L.

旨在研究珊瑚共附生可培养真菌多样性。运用稀释平板法和基于ITS-rDNA基因序列的系统发育分析对珊瑚共附生可培养真菌多样性进行研究,将所得到的基因序列与NCBI数据库GenBank中的序列进行相似性比较并构建系统发育树。实验中从丛生盔形珊瑚表面和内部共分离得到19株菌落形态各异的真菌。ITS-rDNA序列分析及形态学鉴定表明,丛生盔形珊瑚共附生真菌主要包括曲霉菌Aspergillus sp.、枝孢霉菌Cladosporium sp.、炭角菌Xylariales sp.、青霉菌Penicillium sp.、葡萄穗霉菌Stachybotrys sp.、赤霉菌Gibberella moniliformis、镰刀霉菌Fusarium sp.等。分离得到的菌株中,4-13与GenBank中已报道的基因序列的相似性仅为89%。结果表明,与丛生盔形珊瑚共附生的可培养真菌较为丰富,是潜在的新的微生物菌种资源,具有进一步研究的价值。

To study the diversity of cultured symbiotic fungi isolated from Galaxea fascicularis L. in the South China Sea. By dilution plate technique and ITS-rDNA sequence analysis to research the diversity of the cultured symbiotic fungi with the coral, determined sequences data were submitted to GenBank and compared with those known sequences. Phylogenetic tree were built up with MEGA 4.0 program. 19 different morphological strains were isolated from surface and inner of the coral Galaxea fascicularis. ITS-rDNA sequences analysis showed that, symbiosis cultured fungi mainly included Aspergillus sp., Cladosporium sp., Xylariales sp., Penicilliurn sp., Stachybotrys sp., Gibberella moniliformis, Fusarium sp. etc. The similarity of strain 4-13 sequence to the most closely related sequences in GenBank was only 89%. It is concluded that there are diverse fungal symbionts with coral Galaxea fascicularis, which have potential new microorganism resources, deserved for further study.