胰腺癌血清相关蛋白质分子的差异表达

Differential expression of serum.related proteins in pancreatic carcinoma

目的利用双向差异凝胶电泳（2D—DIGE）的方法建立胰腺癌、慢性胰腺炎和正常对照人群外周血清的差异蛋白质双向凝胶电泳图谱并分析差异蛋白质点。方法双向差异凝胶电泳分离20例胰腺癌患者、10例慢性胰腺炎患者和20例正常对照组人群外周血清蛋白，比较不同血清中蛋白质表达的差异。结果成功建立胰腺癌、慢性胰腺炎和正常人之间的外周血清蛋白质双向差异凝胶电泳图谱，软件分析共发现了168个明显差异表达的蛋白质点，其中在胰腺癌组与正常对照组的比较中有22个蛋白质点在胰腺癌血清中表达上调，29个蛋白质点下调；在胰腺癌组和慢性胰腺炎组的比较中有24个蛋白质点在胰腺癌血清中表达上调，54个表达下调；在慢性胰腺炎组和正常对照组的比较中有20个蛋白质点表达上调，19个蛋白质点表达下调。结论双向差异凝胶电泳是快速有效的分离蛋白质新的方法，得到的双向差异凝胶电泳图谱以及显著表达差异的蛋白质点为质谱鉴定提供了实验基础。

Objective To analyze and compare the proteomes expressed by human pancreatic carcinoma, chronic pancreatitis and normal control groups. Methods Differencial expression of the proteomes in peripheral serum was analyzed by fluorescence 2-D difference gel electrophoresis （2D-DIGE）. Results 2D-DICE protein maps in 20 patients with pancreatic cancer, 10 patients with chronic pancreatitis and 20 normal controls were analyzed and 168 spots were identified by gel-analysis software. Between pancreatic cancer group and normal control group 22 protein spots were up-regulated and 29 spots down-regulated; 24 spots were up-regulated and 54 spots down-expressed between pancreatic cancer group and chronic pancreatitis group; 20 spots were up-regulated and 19 spots down-regulated between chronic pancreatitis group and normal control group. Conclusion 2D-DIGE was a rapid and efficient method to separate proteins. 2D- DIGE protein maps and different protein spots would provide an exprimental basis for the phase of mass spectrometry.