摘要
目的观察骨形态发生蛋白-2(BMP-2)基因对兔骨髓基质细胞(MSCs)向软骨细胞分化mRNA表达的影响。方法从兔骨髓细胞中分离提取BMP-2mRNA,构建重组BMP-2真核表达质粒,并转染兔MSCs,采用荧光定量逆转录一聚合酶链反应(RT.PCR)方法检测各组MSCs内Ⅱ型胶原和蛋白多糖mRNA表达水平。结果转染后2,4d实验组MSCs内的Ⅱ型胶原和蛋白多糖mRNA表达量28.7±4.0、236.7±48.5及26.9±4.3、208.2±36.7,均明显高于转染后2,4d的空白对照组(16.1±2.8、99.2±24.8及14.6±2.7、111.1±18.9)和空载体对照组(14.6±2.6、85.4±24.7及16.1±2.8、98.0±22.5)(P〈0.05)。结论重组真核表达载体BMP-2质粒能够诱导MSCs向软骨细胞分化。
Objective To observe the effect of bone morphogenetic protein-2 (BMP-2) on gene expression during the differentiation of marrow stromal cells (MSCs) into chondrocytes. Methods The BMP-2 mRNA was extracted from the rabbit bone marrow cells. The recombinant BMP-2 eukaryotic expression plasmids were constructed and transfected into MSCs. The mRNA expression of type Ⅱ collagen and proteoglycan was detected by using quantitative polymerase chain reaction (PCR) technique. Results At 2rid, and 4th day after transfection of plasmid pEGFP-C3-BMP-2 into MSCs, the mRNA expression levels of type Ⅱ collagen and proteoglycan in MSCs of experimental group were significantly higher than controls (P 〈 0. 05 ). Conclusion Recombinant eukaryotic expression plasmid pEGFP-C3-BMP-2 can induce MSCs differentiation towards chondrocytes.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第8期1370-1372,共3页
Chinese Journal of Experimental Surgery
基金
基金项目:河南省医学高新技术发展扶持项目(2006027)