骨形态发生蛋白-2对兔骨髓基质细胞向软骨细胞分化基因表达的影响

Effect of bone morphogenetic protein-2 gene on genes during differentiation of rabbit marrow stromal cells into chondrocytes

目的观察骨形态发生蛋白-2（BMP-2）基因对兔骨髓基质细胞（MSCs）向软骨细胞分化mRNA表达的影响。方法从兔骨髓细胞中分离提取BMP-2mRNA，构建重组BMP-2真核表达质粒，并转染兔MSCs，采用荧光定量逆转录一聚合酶链反应（RT．PCR）方法检测各组MSCs内Ⅱ型胶原和蛋白多糖mRNA表达水平。结果转染后2,4d实验组MSCs内的Ⅱ型胶原和蛋白多糖mRNA表达量28．7±4．0、236．7±48．5及26．9±4．3、208．2±36．7，均明显高于转染后2,4d的空白对照组（16．1±2．8、99．2±24．8及14．6±2．7、111．1±18．9）和空载体对照组（14．6±2．6、85．4±24．7及16．1±2．8、98．0±22．5）（P〈0．05）。结论重组真核表达载体BMP-2质粒能够诱导MSCs向软骨细胞分化。

Objective To observe the effect of bone morphogenetic protein-2 （BMP-2） on gene expression during the differentiation of marrow stromal cells （MSCs） into chondrocytes. Methods The BMP-2 mRNA was extracted from the rabbit bone marrow cells. The recombinant BMP-2 eukaryotic expression plasmids were constructed and transfected into MSCs. The mRNA expression of type Ⅱ collagen and proteoglycan was detected by using quantitative polymerase chain reaction （PCR） technique. Results At 2rid, and 4th day after transfection of plasmid pEGFP-C3-BMP-2 into MSCs, the mRNA expression levels of type Ⅱ collagen and proteoglycan in MSCs of experimental group were significantly higher than controls （P 〈 0. 05 ）. Conclusion Recombinant eukaryotic expression plasmid pEGFP-C3-BMP-2 can induce MSCs differentiation towards chondrocytes.