紫外线诱变选育苜蓿叶象甲高毒力白僵菌

Screening Highly Virulent Strains of Beauveria bassiana to Hypera postica Gyllenhal by UV Mutagenesis

【目的】选育出对苜蓿叶象甲高毒力的白僵菌。【方法】利用紫外线对前期筛选出的白僵菌菌株照射10和30 min进行诱变,选出高几丁质酶突变株,再用诱变前后的白僵菌对苜蓿叶象甲3龄幼虫进行毒力测定。【结果】用诱变前后的白僵菌对苜蓿叶象甲3龄幼虫室内毒力测定:处理9 d后,诱导菌株Bb-10-12校正死亡率为100%,Bb-30-14校正死亡率为77.78%,而未经紫外线照射的野生型菌株校正死亡率为62.97%。【结论】通过室内毒力测定法,获得了经10 min紫外线照射诱变对苜蓿叶象甲更高毒力的1株白僵菌菌株。

【Objective】The purpose of this program was to screen out highly virulent strains of Beauveria bassiana to Hypera postica Gyllenhal.【Method】 Ultraviolet irradiating was used to irradiate Beauveria bassiana strain for 10 min and 30 min in order to select high chitinase mutant,and then 3 stage larvae of Hypera postica underwent the virulence test with the Beauveria bassiana before and after UV mutagenesis.【Result】The results of the test indicated that corrected mortality of the stain Bb-10-12 was 100%,corrected mortality of the stain Bb-30-14 was 77.78%,and corrected mortality of wild type strain was 62.97% in 9 days later.【Conclusion】A more virulent strain Bb-10-12 of Beauveria bassiana to Hypera postica was obtained by UV mutagenesis.