LC-MS/PAD法测定非法染色的中药蒲黄和黄芩饮片中金胺O

Study of Auramine O in Dyed Radix Scutellariae Praepareta and Pollen Typhae by Uplc-Ms/pad

目的:建立快速、准确、高灵敏度的中药饮片中非法用金胺O染色的专属性检测方法。方法:采用LC-MS/PAD法,离子源为ESI源,正离子检测,通过相对分子质量、二级质谱碎片信息、液相色谱保留时间和PAD图谱4方面信息,对蒲黄、黄芩片中金胺O进行定性检测。结果:10批受试中药饮片中均检测到金胺O。结论:该方法选择性强、灵敏度高,可作为判断中药饮片是否经金胺O进行染色处理的有效检测方法。

Objective：To establish a quick,accurate,sensitive method for analysis of auramine O in dyed RADIX SCUTELLARIAE PRAEPARETA and POLLEN TYPHAE by UPLC-MS/PDA.Method： The UPLC-MS/PDA.Methods：was used.Electrospay ionization（ESI） source was applied and operated in the positive ion mode.Auramine O in RADIX SCUTELLARIAE PRAEPARETA and POLLEN TYPHAE were identified according to the spetrum,chromatographic behavior and mass spectral data by comparison with those of reference substance.Results：auramine O were found in 1 batch of RADIX SCUTELLARIAE PRAEPARETA and 9 batches of POLLEN TYPHAE.Conclusion：The method is selective and sensitive and can be used to detect the auramine O in dyed RADIX SCUTELLARIAE PRAEPARETA and POLLEN TYPHAE.