持续光照对艾灸调控类风湿性关节炎大鼠褪黑激素与类风湿性关节炎血浆IL-1β、IL-4水平的影响

Impact on il-1β,IL-4 in the Plasma and Melatonin in Serum of RA Rats by the Moxibustion Treatment

目的:观察持续光照下艾灸对实验性类风湿性关节炎(Rheumatoid arthritis,RA)大鼠足容积、血清褪黑激素、血浆IL-1β、IL-4含量的影响,探讨褪黑激素在艾灸抗炎效应中的地位和作用。方法:以佐剂性关节炎(AA)作为RA疾病模型,艾灸实验性RA大鼠"肾俞"、"足三里"两穴,采用24h光照作为干预研究手段,观测大鼠足容积,采用ELISA双抗夹心法检测血清MT和血浆IL-1β、IL-4含量。结果:大鼠右后足容积,模型组显著高于空白组(P<0.01),艾灸组显著低于模型组(P<0.01),24h光照+艾灸组与艾灸组相比无显著性差异。大鼠血清MT水平,模型组显著高于空白组(P<0.05),艾灸组显著低于模型组(P<0.05),24h光照+艾灸组显著低于艾灸组(P<0.01);血浆IL-1β含量,模型组显著高于空白组(P<0.05),艾灸组显著低于模型组(P<0.05),24h光照+艾灸组与艾灸组无显著性差异;大鼠血浆IL-4含量,模型组略高于空白组但两组间无显著性差异,艾灸组显著高于模型组(P<0.05),24h光照+艾灸组显著高于艾灸组(P<0.01)。结论:艾灸能够有效抑制RA大鼠足肿胀、抑制血浆IL-1水平、升高IL-4水平,具有明确的抗炎作用;MT与RA炎症过程中密切相关,MT对抗炎因子IL-4可能有抑制作用,MT在艾灸治疗RA炎症中可能不是发挥抗炎作用,而是起到致炎作用。艾灸、持续光照可能通过抑制MT分泌,从而促进抗炎因子IL-4分泌而发挥抗炎作用。

Objective：To calculate foot swelling degree of RA rats and investigate the impact of IL- 1β ,IL-4 in the plasma and melatonin in serum of RA rats by moxibustion treatment, to explore the relationship of MT and impact of cytokines balance in rheumatoid arthritis through moxibustion. Methods ： To moxia ＂＇ Shenshu＂＂ Zusanli＂ on the RA rats , used AA as RA model, 24 hours lighting（2501x） as disturbed method, then determinate IL - 1β .IL -4 in the plasma and MT in serum by ELISA after treatment. Results：For the amount of MT in RA serum, model group were obviously increased than normal group （P 〈 O. 05 ）, moxia group were obviously decreased than model group （ P 〈 0.05 ） ）, 24 hours lighting group were obviously decreased than normal group（ P 〈 0. 01 ）. For the amount of foot swelling degree and IL - 1β in RA plasma , model group were obviously increased than normal group（P 〈 0. 01 ）, moxia group were obviously decreased than model group（ P 〈 0. 01 ） ,24 hours lighting group and moxia group have no significant difference （P 〉 0. 05 ）. For the amount of IL -4, model group were obviously increased than normal group（P 〈0. 01 ） ,moxia group were obviously increased than model group（P 〈0. 01 ） ,24 hours lighting group were obviously increased than moxia group（ P 〈 0.01 ）. Conclusion：Melatonin may have opposite effect to the moxibustion though decreased the amount of IL -4 and IL - 1 β/IL -4 balance ;moxibustion may have anti - inflammatory effect though decreased MT and increased IL -4,IL - 1β/IL -4.