番荔枝种子粗多糖抗氧化能力研究

Antioxidant Ability of Crude Polysaccharides from Sugar Apple(Annona squamosa L.)Seeds

为了探讨番荔枝种子多糖的抗氧化能力,将晒干的种子经粉碎匀浆、乙醇回流、减压浓缩、氯仿萃取和甲醇石油醚抽提后得到粗提物,经干燥后配制成不同浓度的溶液,分别以相同浓度的维生素C作对照,测定了番荔枝种子多糖还原力、抗脂质过氧化能力以及清除DPPH·自由基、超氧阴离子自由基(O·-2)和羟基自由基(·OH)的能力。研究结果表明:番荔枝种子多糖具有较强的还原力,对脂质过氧化有良好的抑制作用,同时具有较强的清除DPPH·、O·-2和·OH的能力,说明番荔枝种子多糖具有较强的抗氧化能力,抗氧化性随浓度增大而增强,但其抗氧化能力弱于维生素C。

In order to explore the antioxidant ability of the polysaccharides of sugar apple seeds, different concentrations of solutions were made with the crude extracts by crushing and homogenating dry seeds, ethanol refluxing, reduced pressure concentration, chloroform extraction and methanol petroleum ether extraction. With the same concentration of vitamin C as the control, the reduction ability, the resistance to peroxidation, and the ability to remove DPPH. radicals, superoxide radical （01） and hydroxyl radicals （＇OH） of polysaecharides from sugar apple seeds were determined. The results showed that the polysaccharides exhibited a strong reduction ability, a good inhibitory effect to lipid peroxidation, and a strong ability to remove DPPH- free radicals, superoxide radical （O2-） and hydroxyl radicals （.OH）, and suggested that the polysaccharides of sugar apple seeds had a strong antioxidant ability, and the oxidation resistance was stronger with the increase of concentration. But its antioxidant ability was weaker than vitamin C.