海南花梨木花精油成分分析及其抑菌活性研究

Chemical Composition and Antibacterial Activity of the Essential Oils from Dalbergia odorifera T.Chen

采用GC-MS联用技术检测了海南花梨木鲜花花精油的化学组成及相对含量,并采用苯醚甲环唑作为对照测定了该精油对香蕉枯萎病菌的抗菌活性。花精油中共鉴定出17种成分,包括烷烃类(53.33%)、邻苯二甲酸二丁酯(14.02%)、邻苯二甲酸二异辛酯(4.37%)、9,12-十八碳二烯酸(14.00%)、α-香树精(8.37%)、1-三十烷醇(2.57%)和二十八烷醇(1.45%)等。抑菌试验表明,花精油和苯醚甲环唑对香蕉枯萎病菌具有一定的抑制作用,其抑制作用随着处理浓度的加大而增强;在接种培养初期,苯醚甲环唑对香蕉枯萎病菌产生明显的抑制作用而花精油不明显,随着时间的延长,花精油对香蕉枯萎病菌的抑制作用逐渐变强,并在7d后与苯醚甲环唑的抑制作用无明显差异。

Chemical constituents and their contents of the essential oils from the flower of Dalbergia odorifera T. Chen were examined by GC-MS and then the anti-microbial activities of the essential oils to Fusarium oxysporum f. sp. cubense were tested. Quantitative determination was conducted using area normalization method. There were 17 chemical constituents identified in the essential oils. The major ones of the constituents were alkanes （53.33%）, Dibutyl phthalate （14.02%）, 1, 2-Benzenedicarboxylic acid, diisooctyl ester （4.37%）, 9,12-Octadecadienoic acid （14.00%）, α-Amyrin （8.37%）, 1, 30-Triacontanediol （2.57%）, 1-Octacosanol （1.45%）. The essential oils exhibited antimicrobial activities against Fusarium oxysporum f. sp. cubense. Variance analysis suggested that there was a significant difference of antimicrobial activity between essential oisl and difenoconazole at the beginning after treatment After 7 days, there was no significant difference in antimicrobial activity against Fusarium oxysporum f. sp. Cubense.