摘要
运用酶动力学方法研究了鲢、鳙的蛋白酶和淀粉酶的活性分布。鲢、鳙的蛋白酶活性在肝胰脏中最强,脾脏中也较强,而在胆汁中未测出。该酶活性在肠粘膜的第Ⅱ段最大,第Ⅰ段向第Ⅱ段递增,第Ⅱ段向肠后端递减,但第Ⅴ段的该酶活性比前段要高些。鲢的淀粉酶活性分布规律与上述情况相似,但鳙的淀粉酶活性在肠粘膜各段中由前向后呈递减趋势,并以第Ⅰ段为最大。在相同的器官组织中,鳙蛋白酶活性高于鲢,但差异不显著(P>0.05),而淀粉酶活性则相反。这两种鱼的蛋白酶活性适宜pH为8.0~8.5,淀粉酶活性在pH7.5时最适。在鲢的肠内容物中发现有纤维素酶活性,但在肠粘膜中未检出该酶。
The
activities of protease and amylase of silver carp, Hypophthalmichthy molitrix and bighead
carp, Aristichthys nobilis were researched with the method of enzyme kinetics.Protease activity
in hepatopancreas was higher than that in any other organs in the 2 species.The protease
activity was higher in spleen, but not detected in bile. As for 5-equal sections of the intestine,
the biggest activity of protease was in section . The activity increased from intestine section to ,
and decreased rapidly thereafter,except the activity was higher in section than that in section .
The activity distribution of amylase was similar to that above in silver carp, but in bighead carp,
the amylase activity decreased from section to,with the bigg est in section .As for the same
organ and tissues in both of the 2 species,protease activity in bighead carp were higher than
that in silver carp,but was not significantly different between them (P>0.05). The differences of
amylase activity between the 2 species were contrary to those of protease activities.Protease
activity of the 2 species had an optimum pH ranging from 8.0 to 8.5 and amlase at 7. 5.
Cellulase activity was detected in the gut content,but not in the intestinal mucosa in silver
carp.
出处
《中国水产科学》
CAS
CSCD
1999年第2期14-17,共4页
Journal of Fishery Sciences of China
关键词
鲢
鳙
蛋白酶
淀粉酶
Hypophthalmichthy molitrix ,Aristichthys nobilis,BZ protease, amylase