鲫鱼种群的随机扩增多态DNA与遗传多样性分析

Analysis of genetic diversity of crucian populations using RAPD markers

利用ＲＡＰＤ技术检测了两个不同水域（天津于桥水库和独流碱河）鲫鱼种群的基因组ＤＮＡ的多态性。用４５个１０ｂｐ的引物对实验鱼的基因组ＤＮＡ进行扩增。实验结果经统计分析得出：种群间ＲＡＰＤ标记的相似率为８４．２％，遗传距离为０．１５８，而上述两个种群内的相似率分别为９４．３％和９３．５％，遗传距离为０．０５７和０．０６５。表明这两个鲫鱼种群内的遗传多态性贫乏，而种群间具有一定的遗传多样性。

Intra and interpopulation genetic diversity of crucian (Carassius auratus) from 2 areas (Yuqiao Reservoir and Duliujian River) were detected using RAPD technique. The genomic DNA of each population of crucian was amplified with 45 primers of 10 bp. The statistical analysis based on RAPD patterns indicated that the interpopulation genetic polymorphism was higher than that of intrapopulation. The interpopulation similarity of RAPD bands was 84.2% and genetic distance was 0.158.However,the intra-population similarity of bands in the populations from Yuqiao Reservoir and Duliujian River were 94.3% and 93.5%,respectively,and the corresponding genetic distances were 0.057 and 0.065,respectively.