农业杀虫剂敌百虫对杜氏盐藻的毒性作用

Toxic Effects of Chemical Pesticide Trichlorfon on Dunaliella salina

本文以一种具有环境耐性的单细胞绿藻杜氏盐藻（Dunaliella salina）作为受试生物,检测杀虫剂敌百虫的毒性作用。实验中检测了敌百虫对盐藻细胞生长、β胡萝卜素水平及超氧化物歧化酶（Superoxide dismutase,SOD）和过氧化氢酶（Catalase,CAT）活性的影响。当敌百虫浓度小于0.050 g/L时,细胞的毒性响应与对照样类似。当使用0.075~0.100 g/L敌百虫处理盐藻,处理初期细胞生长和β胡萝卜素水平降低,一段时间后恢复。当敌百虫浓度大于0.125 g/L,细胞生长和β胡萝卜素水平都会一直降低直至检测不到。敌百虫对杜氏盐藻的10-d IC50是0.179 g/L。实验发现在低浓度下敌百虫会刺激CAT活性的增长。

Dunaliella salina,a unicellular green alga of environmental tolerance,was employed as test organism to investigate the toxicity effects of a pesticide trichlorfon.The influences of trichlorfon on cell growth,β-carotene level and activities of superoxide dismutase（SOD） and catalase（CAT） were investigated.At the concentrations less than 0.050 g/L trichlorfon,cell responses were similar to that of the control.When treated with 0.075~0.100 g/L trichlorfon,cell growth and β-carotene levels declined at first and then revived.When concentrations were higher than 0.125 g/L trichlorfon,both cell growth and β-carotene levels decreased until they were undetectable.The 10-d IC50 of trichlorfon on D.salina was 0.179 g/L.Trichlorfon could stimulate the increase of CAT activity at a low concentration.