产溶菌酶白色链霉菌的筛选及鉴定

Screening and Identification of a Lysozyme-producing Streptomyces albus Strain

从广西各地土壤中取样,利用添加金黄色葡萄球菌的LB培养基初筛,然后用含金黄色葡萄球菌的营养盐平板复筛,所得突变株经摇瓶发酵后测定其用蛋白酶K处理前后的粗酶液活力变化,筛选到一株溶菌酶产生菌RJ-36b。通过菌株形态学和16S rDNA保守序列分析,鉴定其为白色链霉菌。对其发酵条件的初步研究表明,在32℃、200 r/min发酵60 h时粗酶液活力最大,达到108 U/mL。

A lysozyme-producing strain was obtained from soil using the LB plate and PBS plate both contained Staphylococcus aureus,followed by detecting activity changes after treatment with protease K.Through analysis of physiological properties and the sequence of 16S rDNA,it was finally classified into Streptomyces albus sp.RJ-36 b.Results suggested that its enzyme-producing process was closely relevant to its growth.The maximal activity（108 U/mL） was achieved in shake flask at 32 ℃,200 r/min for 60 h.