白附片提取物对酪氨酸酶活性的影响

Effect of White Slice Extracts on Activativity of Tyrosinase

利用超声辅助乙醇提取白附片中活性组分,跟踪粗提物浓度对酪氨酸酶活性的影响规律,发现粗提物能激活酪氨酸酶活性,激活率高达81.82%。利用D101大孔树脂对粗提物进行分离,对酪氨酸酶激活作用最强的是水和30%乙醇洗脱部分(激活率分别是252.00%和326.79%),能显著缩短酪氨酸酶催化单酚反应的滞迟时间。应用反相高效液相色谱(RP-HPLC)分析了各大孔树脂分离组分,发现这些组分在RP-HPLC图谱中前5 min洗脱峰的峰面积越大,其对酪氨酸酶活性的激活率越高,表明白附片中能激活酪氨酸酶活性的物质为极性较大的成分。

Active compounds were extracted from white slice by ethanol aqueous assisted with ultrasound and its activation effect on tyrosinase was traced.It was found that crude extract could activate tyrosinase remarkably,with an activation rate up to 81.82%.The crude extract was separated on a D101 macroporous resin column,the most effective compounds was in water and 30% enthonal eluting fractions（inhibition rate was 252.00% and 326.79%,respectively）,which can greatly shorten the lag time in tyrosinase catalytic reaction while monophenol as substrate.The fractions were subsequently isolated by RP-HPLC method.The area of the peaks which are eluted by RP-HPLC in the first five minutes was larger and the activation rate to tyrosinase was greater.It was indicated that the compounds activating tyrosinase in white slice had a high polarity.