抗生素分解剂β-内酰胺酶的检测方法研究

Development of a Detection Method for β-lactamase as Antibiotic Destroyer in Milk

本研究是利用β-内酰胺酶对青霉素G的特异性分解作用以及舒巴坦对β-内酰胺酶活性有抑制作用原理,按比例添加青霉素G、抑制剂舒巴坦、β-内酰胺酶和被测物,根据添加有一定浓度的藤黄微球菌琼脂平皿上所产生的抑菌圈大小,建立了该酶的检测方法进而对非法添加和残留有β-内酰胺酶的食品进行定性检测。该方法在乳制品中对β-内酰胺酶的检出限为0.004 U/mL。青霉素G的终浓度选择为0.5μg/mL,舒巴坦的终浓度选择为25μg/mL,β-内酰胺酶的终浓度选择为0.04 U/mL。

A detection method of ＂antimicrobial destroyer＂ β-lactamase in milk samples were developed to analyze the active component of antimicrobial destroyer,The qualitative detection of β-lactamase residue in food was based on the inhibition zone diameter.The detection limit of the developed method was 0.004 U/mL β-lactamase in milk samples.