白术茯苓汤不同配伍比例对克罗恩病大鼠细胞因子的影响

Effects of Baizhu Fuling Tang in Different Proportioning on Crohn's Disease Rats' Cytokine

目的:考察白术茯苓汤1:1、2:1、1:2三种配比对克罗恩病大鼠细胞因子的影响。方法:采用Morris方法复制克罗恩病动物模型,白术茯苓汤不同配比灌胃给药,酶联免疫吸附法(ELISA)检测大鼠血清肿瘤坏死因子(TNF-α)、白介素-1β(IL-1β)、白介素-4(IL-4)、白介素-10(IL-10)含量。结果:与空白组相比,模型组TNF-α、IL-1β明显升高(P<0.05),IL-4、IL-10无明显变化;与模型组相比,1:1组明显降低TNF-α水平(P<0.05);1:2组明显降低IL-1β水平(P<0.05);2:1组能同时降低TNF-α、IL-1β水平(P<0.05);3种配比对IL-10无明显调节作用,但均能恢复IL-4至正常水平,其中以2:1组明显。结论:白术茯苓汤2:1组对TNF-α、IL-1β调节作用优于1:1组和1:2组,表明白术茯苓汤不同配比对Th1/Th2免疫失衡影响不同。

Objective：Researching the effect of Baizhu Fuling Tang 1：1,2：1,1：2 proportioning on Crohn’s disease rats’ cytokine.Methods：Using internationally recognized Morris’ methods to build the rats model and curing CD rats by Baizhu Fuling Tang in different proportioning,using ELISA kit to determine the TNF-α,IL-1β,IL-4,IL-10 in rats blood serum.Results：Comparing to the blank group：TNF-α,IL-1βlevel distinctly ascend（P〈0.05）,IL-4,IL-10 level are no significant difference;compa-ring to the model group,1：1 group could reduce TNF-αlevel remarkably（P〈0.05）;1：2 group could reduce IL-1βlevel remarkably（P〈0.05）;2：1 group could reduce TNF-αlevel and IL-1βlevel simultaneously（P〈0.05）;three proportioning group have no adjusting effects on IL-10;but which could make IL-4 back to normal over time,the more obvious is 2：1 group.Conclusion：The adjusting effects of Baizhu Fuling Tang 2：1 proportioning group is better than that of 1：1 and 1：2 ratios group in TNF-α and IL-1β level,which show different proportioning of Baizhu Fuling Tang have different effect on imbalanced immunity of Th1/Th2.