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当归散对低容受性小鼠子宫内膜IL-1β表达的影响及相关模型中医内涵探讨 被引量:7

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摘要 目的:观察促排卵所致低容受性小鼠子宫内膜的变化及其中医辨证特性,探讨当归散对子宫内膜中IL-1β表达的影响。方法:采用曲普瑞林+HMG+HCG长周期促排卵方法制备小鼠低容受性子宫模型,造模同时分别预防性给予当归散、当归-黄芩以及当归散去当归-黄芩供试液,分别于排卵后24h和48h分离子宫,观察各组子宫内膜形态变化,并测定内膜中IL-1β表达水平。结果:(1)子宫内膜形态:模型组子宫内膜增厚,间质发育异常,血管发育不良,与正常组差异显著。当归散组及归-芩组内膜偏厚,但血管发育较好,与正常组相近;去归-芩组与模型组接近。(2)IL-1β表达:排卵后24h,模型组IL-1β表达与正常组无明显差异;归-芩组IL-1β值低于正常组水平(P<0.05)。48h时,当归散组IL-1β值无明显变化,其余各组表达均较24h时显著升高(P<0.05或0.01);其中模型组和去归-芩组IL-1β表达上调明显,高于正常组(P<0.05);当归散组IL-1β值显著低于模型组(P<0.05),与正常组无差异。结论:促排卵药物引起的动物子宫内膜发育不良,其显微变化与中医血虚、血瘀及热证病变吻合;当归散能改善造模引起的子宫内膜发育不良;当归散能调节子宫内膜的IL-1β的适度表达;方中当归-黄芩配伍体现了当归散和血清热的核心立法思想。
出处 《辽宁中医药大学学报》 CAS 2011年第8期92-94,共3页 Journal of Liaoning University of Traditional Chinese Medicine
基金 国家自然科学基金资助项目(30801463)
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参考文献9

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二级参考文献19

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