HLA-DRB1基因分型在移植配型及亲子鉴定中的应用

HLA-DRB1 allele detection applied in transplaning typing and consanguinity identify

应用聚合酶链反应－序列特异性引物（ＰＣＲ－ＳＳＰ） 方法对南京地区汉族人群ＨＬＡ－ＤＲＢ１ 位点基因特异性及骨髓移植配型、亲子鉴定等进行了分析。ＤＲＢ１ 基因频率范围在０．００３３～０．１８３３之间， 以ＤＲ９、ＤＲ４占多数； 骨髓移植通过配型移植成功３ 例， ２ 例存活情况良好； ＤＲＢ１ 位点排除亲子关系５例， 其中２ 例为ＨＬＡ－ＤＲＢ１单独排除亲缘关系。本法具有操作简单、快速、结果可靠的特点， 不仅适用于移植配型、法医学亲子鉴定和个人识别，

HLA DRB1 specific Allele,bone marrow transplant typing and consanguinity identifying were analyzed for the groups of Han nationality in Nanjing by the method of polymerase chain reaction sequence specific primer(PCR SSP).DRB1 gene frequency of the groups is between 0 0033~0 1833 DR9 and DR4 have a larger proportion than others.Bone marrow transplanting has succeeded in three cases through transplanting typing,and two of the them were alive with smooth condition.DRB1 site can identify 5 cases non consanguinity,two of them non consanguinity were identified with HLA DRB1.This method is simple,fast,accurate.Its result is available.It can not only apply to transplant typing,consanguinity identifying in medical jurisprudence and individual identifying but also research associated disease and human heredity genetics.