光谱法研究铜锌超氧歧化酶与组氨酸钴(Ⅱ)的相互作用 Ⅲ.溶液的pH值、作用时间的影响

Study on the Interaction of Copper Zinc Superoxide Dismutase with Cobalt(Ⅱ) Histidine by Spectral Analysis Ⅲ Effects of pH,Interaction time

采用ＶＩＳ，ＩＣＰ 及酶活性测定等方法，研究铜锌超氧歧化酶（Ｃｕ２Ｚｎ２ＳＯＤ）与组氨酸钴（Ⅱ） （Ｃｏ（Ｈｉｓ）ｎ ） 直接相互作用时溶液ｐＨ值、作用时间对此类相互作用的影响。结果发现，溶液的ｐＨ值越高，作用时间越长。

The direct interaction of Cu 2Zn 2SOD with organic metal compound (Cobalt(Ⅱ) Histidine) was studied by ICP,VIS and the measurement of enzyme activity,and also investigated the effect of pH values and different interaction times for this interaction.The results showed that with the increased in pH values and the more longer interaction times,the intensity of interaction were increased and the corresponding catalytic activity of enzyme were affected.