鳄鱼血SDS-PAGE鉴别方法的研究

Distinguish crocodile blood by SDS-PAGE

目的:建立鳄鱼血SDS-PAGE鉴别方法。方法:利用电泳缓冲液制备鳄鱼血、绵羊血、豚鼠血、鸡血、兔血、鹿血等6种动物全血样品液,采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法,对6种动物全血进行鉴别研究。结果:以7.5%～11%的梯度分离胶、60μg蛋白量上样,大板(16 cm×20 cm)进行SDS-PAGE鉴别,6种动物全血的电泳谱带有明显差异,鳄鱼血存在特征条带。结论:本方法灵敏度高、专属性强,可作为鳄鱼血与其他动物血的鉴别依据。

Objective：To establish the SDS-PAGE identification method for crocodile blood.Methods：Electrophoresis buffer was used to prepare six animals＇ whole blood sample solution.The animals were：crocodile,sheep,guinea pig,chicken,rabbit,and deer.Sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE） method was used to identify six animals＇ whole blood.Results：The best SDS-PAGE conditions to get good resolution were 60 μg protein sample loaded to each well and using the 7.5%-11% gradient separating gel plate（16 cm×20 cm） to run the samples.The obviously different electrophoresis bands of six animals＇ whole blood were obtained by the method and crocodile blood has the characteristic bands.Conclusion：This method is sensitive and could be used easily to distinguish the crocodile blood from other five kinds bleed.