紫外诱变和氨基酸抗性筛选选育那西肽高产菌株

Breeding high-yielding strains of nosiheptide with UV mutagenesis and amino acids resistance screening

目的筛选出那西肽高产菌株。方法以活跃链霉菌CB040517作为出发菌株,通过紫外-氯化锂诱变处理,并结合前体复合氨基酸抗性筛选,选育那西肽高产菌。结果通过致死率和正突变率的考察,确定紫外最佳诱变剂量为100s。在分离培养基上层加入氯化锂和底层加入复合氨基酸进行正突变株的定向筛选,选育得到那西肽高产菌株UV-19-A12,其摇瓶效价达904.00μg/mL,比出发菌株提高72.5%,经过五代斜面传代试验考察,该菌株遗传性状稳定。结论紫外诱变和氨基酸抗性筛选可以获得那西肽高产菌株。

Objective To screen out Streptomyces actuosu strains of high yield of nosiheptide. Methods UV mutagenesis and prosoma compound amino acids were used to screen high-yielding strains of nosiheptide directionly by using Streptomyces actuosu CB040517 as orginal strain. Results The results showed that the dose of UV irradiation was determined 100s in term of death rate and positive mutation. Using isolation medium with lithium chloride on the upper layer and compound amino acids on the bottom layer, a high-yield nosiheptide-producing strain named UV-19-A21 was obtained, whose shaking production arrived to 904μg/mL, increased by 72.5% than the orginal strain, its character was satable after five generations. Conclusion The Streptomyces actuosu strains of high yield of nosiheptide could be obtained by UV mutagenesis and prosoma compound amino acids resistance screening.