阶跃洗脱吸附层析法分离纯化红霉素A

Separation and purification of erythromycin A by stepwise elution adsorption chromatography

红霉素碱粗品经大孔吸附树脂SP825二级阶跃洗脱层析法分离纯化红霉素A。研究了红霉素A、C组分的固定床吸附、洗脱过程,包括穿透曲线、洗脱曲线的测定及洗脱条件的选择等。结果表明,红霉素A组分在竞争吸附过程中对红霉素C组分具有一定优势,采用乙酸乙酯洗脱对红霉素A组分的选择性和洗脱率均较好。为了进一步提高分离效率,进行了柱层析实验,考察了红霉素上柱量和第一阶段洗脱方式等对红霉素A、C组分分离效果的影响。优化的红霉素上柱量为树脂饱和吸附量的40%左右,依次对层析柱以2%乙酸乙酯水溶液进行第一阶段洗脱5BV以及纯乙酸乙酯进行第二阶段洗脱,所得最终产品中红霉素A组分含量可达95.8%,总收率可达96.1%。此工艺过程简单、高效、经济、无污染。

Erythromycin（EM） A was separated and purified from impure EM with macroporous resin SP825 by 2-stepwise elution chromatography. The fixed-bed adsorption and desorption of EM A and C, including the breakthrough curves, elution curves, and elution conditions were studied in the present paper. The results showed that EM A had an advantage over EM C in competitive adsorption, and ethyl acetate was chosen as the eluent for its high desorption rate and good selectivity of EM A. Column chromatography experiments were also carried out to further improve the separation efficiency of EM A and C. The loading mass of EM and the first step elution mode were determined. The optimal loading mass of EM was about 40% of the saturated adsorption capacity of the resin. By eluting the column with 2% ethyl acetate solution for 5BV（bed volume） in the first step and pure ethyl acetate in the second step in order, the purity of EM A in final product could reach 95.8% with a total yield of 96.1%. This process is simple, efficient, economical and eco-friendly.