皮质发育障碍大鼠模型学习记忆行为和海马长时程增强的研究

Study of learning ＆ memory behaviour and LTP in hippocampal slices of DCDs rat models

目的：研究皮质发育障碍（DCD）大鼠模型空间学习记忆及离体海马长时程增强（LTP）变化，探讨DCD大鼠模型认知功能损伤的机制。方法：建立DCD大鼠模型，采用Morris水迷宫实验对DCD大鼠模型和正常对照组进行空间学习、记忆的行为学检测，应用膜片钳技术研究DCD大鼠模型海马脑片CA1区LTP的改变。结果：Morris水迷宫实验中DCD大鼠与正常对照组相比逃避潜伏期延长，穿过原平台位置次数和在原平台象限探索时间百分率下降；DCD大鼠模型海马脑片CA1区LTP诱出率、幅值增加百分率与正常对照组相比均明显降低[（40％vs100％，P〈0．05；（108±5．6）％vs（132±15．4）％，P〈0．05）]。结论：DCD大鼠模型的空间学习记忆能力降低，海马突触可塑性也发生降低。

Objective：To investigate the changes of spatial learning, memory and hippoeampal long term potentiation（ LTP） in vitro of the disorders of cortical derelopments （DCDs） rat models. Methods： To establish the DCDs rat models. The spatial learning and memory behavior of the DCDs rat models were assessed by Morris water maze. LTP in CA1 region of hippocampal slices of the DCDs rat models were analyzed through patch clamp technique. Results：In the Morris water maze, latency on the hidden platform were significantly longer, the frequency of crossing the platform and the time percentage of swimming in the former platform quadrant significantly decreased in the experimental group. Compared with the control group, the probability of the induction of LTP and average population spike amplitudes of LTP in the CA1 region of the DCDs rat models were significantly decreased（40 % vs 100% ;P〈0.05） ; [（ 108± 5.6） %vs （132± 15.4） %] ;P〈0.05）. Conclusion： The ability of spatial learning and memory of the DCDs rat models decreased. The hippocampal synaptie plasticity of the DCDs rat models also decreased.