插入序列ISAba1及IS1133在鲍氏不动杆菌临床分离株中的分布

Distribution of insertion sequence ISAba1 and IS1133 in acinetobacter baumannii clinical isolates

目的分析插入序列ISAba1及IS1133在鲍氏不动杆菌临床分离株中的分布。方法选择分离自2009年1-6月医院门诊和住院患者的各类临床标本分离出的鲍氏不动杆菌172株,使用PCR和PCR产物测序的方法检测插入序列ISAba1和IS1133在临床分离株中的分布。结果 172株中有159株含有ISAba1,阳性率为92.4%,未检出IS1133序列。结论大部分鲍氏不动杆菌临床分离株都含有插入序列ISAba1,可能与多药耐药密切相关。

OBJECTIVE To investigate the presence of ISAba1 and IS1133 in Acinetobacter baumannii（ABA） clinical isolates.METHODS A total of 172 ABA isolates were collected from Jan to June 2009,PCR screening was carried out to identify possible ISAba1 and IS1133 genes.RESULTS Of 172 strains of ABA,There were 159 strains carrying ISAba1 with positive rate of 92.4%,no IS1133 were detected out in the isolates.CONCLUSION Most of ABA isolates contain ISAba1,which is closely related to multi-drug resistance.